Figure 2 | Scientific Reports

Figure 2

From: Quantification of allelic differential expression using a simple Fluorescence primer PCR-RFLP-based method

Figure 2

Evaluation of fluorescence density of the PCR products amplified with different FAM labeled primers. (A–C) Fluorescence density analysis of serial diluted 1× FAMer or 2× FAMer-labeled primers. (D) The diagram of PCR products with 8 different types of FAM-labeled primers. Green asterisk represents the 6-FAM-labeled nucleotide. (E) A vector containing normal and mutant GAPDH DNA copy was used as PCR template for the fluorescence density analysis. (F) The fPCR-RFLP results of 8 different FAM-labeled primers. (G) The fluorescence density of wild type (wt) and mutant (mt) GAPDH copies revealed that the ratio of wt/mt was approximately 1.0. Full-length gels are presented in Supplementary Fig. 3.

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