Figure 3 | Scientific Reports

Figure 3

From: Quantification of allelic differential expression using a simple Fluorescence primer PCR-RFLP-based method

Figure 3

Assessment of the accuracy of fPCR-RFLP method. (A) The map of two vectors contain normal and mutant GAPDH or SRY gene. (B,C) The fPCR-RFLP results of GAPDH and SRY using the constructed plasmids as template. Lanes #G1~#G7 represent 7 independent colonies of GAPDH gene; lanes #S1~#S7 represent 7 independent colonies of SRY gene. (D) The map of vectors containing A or G alleles of the PAPPA2 gene. (E) The fPCR-RFLP results of PAPPA2 gene with different input ratios of A allele and G allele. (F) The linear range of fPCR-RFLP method analyzed based on the results of PAPPA2 gene. Error bars represent S.D. from n = 3 replicates. (G,H) The results of fPCR-RFLP of PAPPA2 gene and miR-155 using genomic DNA as template. Lanes PA1~PA8 represent 8 independent individuals for PAPPA2 gene; lanes mi1~mi8 represent 8 independent individuals for miR-155 gene; P2 is the positive control of restriction enzyme digestion. Full-length gels are presented in Supplementary Fig. 3.

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