Figure 4
Validation of ADE of PLAG1 and KIT gene using fPCR-RFLP method. (A,B) The expression difference of two alleles of PLAG1 and KIT genes detected by RNA-seq in several pig tissues at different developmental stages. A, adult; E, embryo. (C) The tissue distribution of PLAG1 and KIT genes. 18 s rRNA used as internal control. (D,E) Detection of two ADE genes (PLAG1 and KIT) using fPCR-RFLP from three individuals. Sp, spleen; Mu, Muscle; Br, brain; Li, liver; Ad, adipose. Lanes PL1~PL8 represent the fPCR-RFLP results of PLAG1 gene in 8 independent individuals. Lanes K1~K9 represent the fPCR-RFLP results of KIT gene in 9 independent individuals. P2 is the positive control of restriction enzyme digestion. (F) The ratio of A and G allele of PLAG1 gene at cDNA and DNA level. Data were pooled from D, and t test was used to check statistics. (G) The ratio of T and C allele of KIT gene at cDNA and DNA level. Data were pooled from E, and t test was used to check statistics. Full-length gels are presented in Supplementary Fig. 3.
