Figure 4
From: Interaction of RNA viruses of the natural virome with the African malaria vector, Anopheles coluzzii
Toll pathway activity limits levels of AnCPV in Anopheles coluzzii. Silencing the Toll negative regulator Cactus activates Toll signaling, and decreases the prevalence of AnCPV when analyzed in the AnCV low-infected group of mosquitoes to minimize the interference effect from AnCV. AnCV low infection level was defined as in Fig. 3 as the AnCV Non-infected and Low-infected groups combined. Plots show the prevalence of infection by AnCPV as a proportion, in three biological replicates ((A) replicate 1, (B) replicate 2, (C) replicate 3). dsCactus indicates mosquitoes injected with double-stranded RNA directed against Toll negative regulator Cactus transcript, and dsGFP indicates mosquitoes injected with irrelevant dsRNA. X-axis, dsRNA treatment, y-axis, AnCPV infection prevalence. (n) indicates the total number of mosquitoes for each of three biological replicates. Statistical differences were first tested independently within replicates by the Wilcoxon-Mann-Whitney test using 100,000 permutations to assess the p-value (given above each replicate plot). Individual replicates displayed a consistent direction of change, and consequently individual p-values were combined using the method of Fisher to generate a combined p-value (combined p = 0.0013, chi-square = 21.8, df = 6). Detailed statistics in Supplementary Table S4.
