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Figure 1

From: Short-term 3D culture systems of various complexity for treatment optimization of colorectal carcinoma

Figure 1

CRC spheroid kinetics and morphology. (A) Spheroid diameter of cells 48 hours post-seeding of 1000–4000 cells/well. (B) Growth kinetics represented in spheroid diameter over 7 days at optimized seeding densities of 1000 cells/well for DLD1, SW620, LS174T, HCT116 and HT29, and 1500 cells/well for Caco2. Error bars represent the standard deviation, n = 3–15. (C) Representative images of the CRC spheroids and the healthy colon epithelial cell line (CCD841 CoN) grown at the optimal seeding densities between day 1 and 7. Scale bar represent 200 µm.

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