Figure 2

Epithelial cells in the mesadenial gland/reservoir are binucleated, and their nuclear position relative to the epithelial plane is unaffected by nutrient conditions. (A,C,E) MG. (B,D,F) MR. (A and B) Under feeding once a week for more than two weeks. (C,D) After starvation for 1 month. (E,F) Under nonfeeding conditions (1 day after adult molting). (Square photos in A–F) Surface views. (Vertical rectangular photos in A–F) Longitudinal sectional views that have been reconstituted from the Z-stacks of the confocal images on the yellow dashed vertical lines in the square photos in (A–F). (Horizontal rectangular photos in A–F) Transverse sectional views that have been reconstituted from the Z-stacks of the confocal images on the yellow dashed horizontal lines in the square photos in (A–F). Green: phalloidin staining. Magenta: DAPI staining. Scale bars in (A–F) are 25 μm. Although the epithelial cells along the lateral edge of the MG frequently appear to be in the mononucleate state at a glance, the two nuclei of these cells overlap at this angle of view. Note that the F-actin staining on the plasma membrane by phalloidin in the MRs is not visible because of the strong outer muscle staining. The growth in the size of the cells in the MR can be noted from the distance between the nuclear sets.