Figure 3
From: Binucleation of male accessory gland cells in the common bed bug Cimex lectularius

Autophagy in the mesadenial gland cells under starved conditions. (A,B) Difference in the amount of phalloidin-positive puncta in the MG epithelial cells between the starved (A) and feeding (B) conditions. Green: phalloidin staining. Magenta: DAPI staining. (C–C”’) After 1 month of starvation, the MG was double-stained with phalloidin (green) and LysoTracker (magenta). (C) Low magnification view of the double-stained MG. (C’–C”’) Enlargement of the boxed area in C. (C’) Phalloidin channel. (C”) LysoTracker channel. (C”’) Merged image. Green: phalloidin staining. Magenta: LysoTracker staining. Cyan: DAPI staining.