Figure 6

In Cimex, the angle of the line connecting the two nuclei relative to the proximodistal axis is stable in the mesadenial gland but variable in the mesadenial reservoir. (A,C,D) MG under feeding conditions. (B,E,F) MR under feeding conditions. (A,B) Representative pairs of nuclei with angles of the lines connecting the two nuclei to the proximodistal axis (yellow arrows from proximal to distal) in the MG and MR. Since the small nuclei that are shown to be in a scattered distribution in the MR are in the muscle cells but not in the epithelial cells, they were excluded in the angle measurement. Green: phalloidin staining. Magenta: DAPI staining. Scale bars in A and B are 25 and 100 μm, respectively. (C–F) Rose graphs showing the frequencies of each angle range. (C,E) Under feeding conditions. (D,F) Under starved conditions. The averaged values (parenthesized) and standard deviations (outer red arc) of the angle of the line connecting the two nuclei relative to the proximodistal axis are 76.5 ± 12.0 (N = 179) in (C) and 72.2 ± 15.5 (N = 273) in (D). The P-value between (C,D) is 0.13. Similarly, the averages and standard deviations are 62.1 ± 22.5 (N = 163) in (E) and 55.1 ± 25.1 (N = 153) in (F). The P-value between (E,F) is 0.18.