Figure 2
ApoB derived peptides effects on the Zeta potential of treated bacterial cells. Zeta potential values of B. globigii TNO BM013 (A) and P. aeruginosa PAO1 (C) bacterial strains were determined upon treatment with r(P)ApoBL or r(P)ApoBS peptides at a concentration corresponding to MIC100 values. Zeta potential values of bacterial strains not responsive to antimicrobial ApoB derived peptides, i.e. S. aureus MRSA WKZ-2 (B) and P. aeruginosa ATCC 27853 (D), were determined upon treatment with peptides at the highest concentration tested (40 µM) for 180 min. Dashed curves represent the Zeta potential values of ApoB derived peptides alone in bacterial culture medium (NB 0.5X). In the case of B. globigii TNO BM013 (A) and P. aeruginosa PAO1 (C) bacterial strains, a positive ζ shift is indicative of an interaction between bacterial cells and peptides. On the contrary, in the case of S. aureus MRSA WKZ-2 (B) and P. aeruginosa ATCC 27853 (D) bacterial strains, signals recorded upon treatment with peptides (continuous lines) are almost completely superimposable to those recorded for free peptide in culture medium at 40 µM (dashed lines), thus indicating absence of interactions between bacterial cells and peptides. Data represent the average of at least three independent experiments.
