Figure 6
From: Neuralized family member NEURL1 is a ubiquitin ligase for the cGMP-specific phosphodiesterase 9A
NEURL1 promotes degradation of PDE9A in primary neurons. PDE9A-V5His was expressed either alone or together with NEURL1-Flag in primary hippocampal (HC), cortical (CTX) or cerebellar (CBL) neurons. 2–3 days post transfection, the cells were lysed and subjected to immunoblotting with the indicated antibodies. NEURL1-Flag could not be unequivocally detected due to the emergence of high background signal in primary neurons when using anti-Flag antibodies (data not shown). Immunoblotting with anti-GAPDH antibody was performed to demonstrate equal loading. IB, immunoblot.
