Figure 3 | Scientific Reports

Figure 3

From: Oxygen dissociation from ferrous oxygenated human hemoglobin:haptoglobin complexes confirms that in the R-state α and β chains are functionally heterogeneous

Figure 3

Average time course of O2 dissociation from Hp1-1:Hb(II)-O2 (panels A and C) and from Hp2-2:Hb(II)-O2 (panels B and D) by mixing the Hp-:Hb(II)-O2 solutions with the CO solution in the presence of dithionite (panels A and B, respectively) and with the dithionite solution only (panels C and D). The continuous lines were calculated according to Eq. 2 with the following parameters: (panel A) a = 0.49, koff1 = 27.4 s−1, b = 0.51, and koff2 = 15.1 s−1; (panel B) a = 0.47, koff1 = 26.3 s−1, b = 0.53, and koff2 = 13.1 s−1; (panel C) a = 0.52, koff1 = 28.3 s−1, b = 0.48, and koff2 = 14.2 s−1; and (panel D) a = 0.49, koff1 = 28.8 s−1, b = 0.51, and koff2 = 12.6 s−1.

Back to article page