Figure 1

Liver macrophage flow sorting schematic. CD45 was used to select for myeloid cells. CD11b and SiglecF were used to gate out eosinophils (Eos, CD11b⁺ SiglecF⁺) from macrophages (Mϕ, CD11b⁺ SiglecF⁻). Macrophages F4/80 and CD11b were used to identify infiltrating macrophages (IM, CD11b^hi F4/80^Int) from Kupffer cells (KC, CD11b^Int F4/80^hi). Mixture of V450 conjugated anti-Ly6G, CD3, CD19, NK1.1 were used to gated out the neutrophils, lymophocytes and Nature Killer cells. IM were finally separated into the two infiltrating macrophage phenotypes based on expression level of Ly6C: Ly6C^hi and Ly6C^low.