Figure 2

TRPV4 expression in WT and Trpv4 KO mice. (a) TRPV4 expression in the circumvallate papillae. (b) Representative images of haematoxylin and eosin staining of WT and Trpv4 KO. (c) Double labelling experiments of TRPV4 (green) with NTPdase2, PLCβ2, CAR4, 5-HT, Sonic hedgehog, and keratin 14 (red) in the circumvallate papillae. Scale bars = 50 μm (a,b) and 20 μm (c). Arrows indicate colocalization of signals of TRPV4 and keratin 14.