Figure 1
From: In utero infection of Zika virus leads to abnormal central nervous system development in mice
ZIKV infection during pregnancy induces mouse brain development disorder after birth. ZIKV (500 PFU) and mock infection were conducted via in utero at E13.5. (A) E17.5 embryos morphology post- ZIKV and mock infection. (B) Brain images at E17.5. (C) brain weights at E17.5 (n = 3 per group). (D,E) ZIKV detection in the embryo cortex by immunostaining (D) and qPCR (E, n = 3–4 per group). Scale bar: left panels, 50 µm; enlarged images in the right panels, 10 µm. (F) Pups at P3, P9 and P30 and (G) changes in their body weights post-ZIKV infection (n = 5, ZIKV 500 PFU) at different postnatal time points. (H) Brain image and (I) brain weight at P9 after ZIKV infection (500 PFU, n = 5–6 per group). (J) Brain images and (K) brain weight at P30 after ZIKV infection (500 PFU, n = 6 per group). *P < 0.05, **P < 0.01, ***P < 0.001, calculated by Student’s t-test. NS, not significant. Graphs indicate mean ± SD.
