Figure 4
WKYMVm improved alveolarization, pulmonary vascular remodelling and angiogenesis in hyperoxic mice. (a,b) Representative photomicrographs of mice lungs stained with haematoxylin and eosin at a magnification of 200× and 400×, respectively (scale bar, 100 µm). (d,e) Morphometric evaluation of mean linear intercept (MLI) and mean alveolar volume (MAV), respectively. (f) Pulmonary vascular remodelling measured as a percentage of medial wall thickness. (c and g) Representative photomicrographs of von Willebrand factor (vWF, green) and its light intensity per high-power field (HPF), respectively. Cell counter-staining was performed using 4′,6-diamidine-2′-phenylindole dihydrochloride (DAPI, blue). Images were taken at a magnification of 100× (scale bar, 100 µm). Data are presented as mean ± SD. *P < 0.05 vs. normal control (NC). #P < 0.05 vs. hyperoxia control (HC). PA = pulmonary artery. AW = airway.
