Figure 4

Hyperphagia contributes to obesity in Stim-TRiPI in adult male flies. (A) Stim-TRiPI On results in hyperphagia of male flies from Stim-TRiPI On induction onwards compared to Stim-TRiPI Off flies. Note that Stim-TRiPI On control adult male flies also showed a transient increase of food intake during the induction period, which returned to the same level as the corresponding Stim-TRiPI Off control flies at day 1 (n = 34–48). (B) Average daily food intake increases over a period of 10 days of Stim-TRiPI On compared to the Stim-TRiPI Off flies. (C) Pair-feeding reduces but not fully suppresses obesity development in Stim-TRiPI On male flies over a period of 11 days when compared to ad libitum fed flies (n = 4–8). Relative (Rel.) fat content is represented as fold change, which refers to the basis value (=1) obtained from corresponding Stim-TRiPI Off flies. Data are presented as means ± standard deviations. All data were analyzed by the two-tailed unpaired Student’s t-test. No *p ≥ 0.05, *p < 0.05, **p < 0.01, ***p < 0.001.