Figure 4

Overexpression of BrALDH7B2 in pET28a in E. coli: (a) Spot assay showed better tolerance of recombinant BL21/BrALDH7B2. (b,c) Growth kinetics study of BL21/pET28a and BL21/BrALDH7B2 during abiotic stress. Bacterial cells were grown in LB medium having 400/500 mM NaCl or 5/6 mM H2O2 treatments. Cells were monitored for 10 h at 1 h interval at 37 °C. Mean values and standard deviations of growth of three independent experiments were plotted in the form of graph.