Figure 5
Heterologous expression of BrALDH7B2 in yeast mutant W3O3-1-A. (a) Comparison of cell survival assays of S . cerevisiae mutant W3O3-1-A expressing PYES2/NTA and PYES2/NTA: BrALDH7B2 under different abiotic stress treatments up to 10−3 fold dilutions. W3O3-1-A cells at OD600 = 0.5, were spotted on YPDA medium (Yeast potato dextrose agar) supplemented with 400/500 mM NaCl and 5/6 mM H2O2 and 2% galatose. (b) Pictorial depiction of PI treatment. Images were observed under Laser scanning confocal fluorescence microscopy of yeast cells carrying PYES2/NTA and PYES2/NTA: BrALDH7B2 in 5 mM and 6 mM H2O2 at λem = 620 nm and λexc = 530 nm. Dead cells were stained red colour.Scale bar = 20 μm; (c) Quantification of dead cells under confocal microscopy in each field. Three different fields of view from each slide were chosen randomly. More than 300 cells from three independent experiments were counted and plotted in the form of graph.
