Figure 3
HO53 and HO56 alter expression of cytokines in BCi cells. Expression of (a) CXCL8 and (c) TNF at mRNA level was analyzed by qRT-PCR and normalized to TUBB (tubulin-β) reference gene. The secretion of the proteins (b) IL8 (CXCL8) and (d) TNFα was evaluated by ELISA in corresponding cell culture medium. Analysis was performed at 24 h (black bars) and 48 h (grey bars) after stimulation with Entinostat (10 μM), HO53 (75 μM) and HO56 (75 μM). For all experiment each bar represents mean value of 3 independent experiments ± SEM; statistical significance was calculated in comparison to the untreated control cells (Ctrl) using two-way ANOVA with Dunnett’s multiple comparisons test; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Significant changes are highlighted.
