Figure 8
From: TGFβ-induced cytoskeletal remodeling mediates elevation of cell stiffness and invasiveness in NSCLC
siRNA-mediated knockdown of MYH15 and MYL9, but not MYLK and TPM1 reduces TGFβ-induced stiffening of H1975 cells. (A–D) Creep-and-recovery curves of H1975 transfected with either non-targeting siRNA or siRNA specific to one of the candidate genes for 36 h followed by stimulation with 2 ng/ml TGFβ for 24 h. Afterwards cells were trypsinized and injected into the microfluidic system of optical stretcher. MYH15 siRNA-silenced H1975 cells exhibit the largest softening in comparison to control probes among four tested candidate genes. (E) TGFβ-induced 3D collagen gel invasion of H1975 cells upon siRNA-mediated MYH15 knockdown. Cells were siRNA-transfected and seeded on collagen gels, allowed to adhere overnight and stimulated with 2 ng/ml TGFβ for 3 days. Afterwards cells were fixed, stained with Hoechst and imaged on confocal microscope (Zeiss LSM710). n inside the boxes indicates the number of cells measured per condition, n outside the boxes corresponds to the number of independent repetitions. **p < 0.01 and ***p < 0.001 significance levels are according to one-way ANOVA.
