Figure 2

Schematic representation of the identified transgenic cassette of the tested GM petunia plants, based on the sequence information generated in this study. (A) The petunia part is represented in green while the transgenic cassette is indicated in grey. The approximate size of the transgenic elements is indicated in base-pairs (bp). The beta-lactamase gene (bla); the Cauliflower Mosaic Virus (CaMV) 35S promoter (p35S); the maize A1 gene (A1). The starting positions and walking directions of the applied DNA walking methods anchored on the p35S element are indicated by arrows (Table 1). The positions of primers (Junction-F and Junction-R) used to confirm the observed transgene flanking region with the MinION platform are indicated by arrows (Table 1). The consensus sequences of the transgene flanking region confirmed by PCR amplification and Sanger sequencing for the petunia samples n°1–18, 21–23 (see Supplementary File 5) is indicated. (B) The consensus sequences produced by the MinION platform with the DNA walking p35S-F (see Supplementary Files 3–4) and the DNA walking p35S-R (see Supplementary File 2) methods applied on all GM petunia samples are indicated. In all these sequences, the petunia part is indicated in lower case while the transgenic cassette, containing bla (underlined), uncharacterized vector part, p35S (in bold) and the maize A1 gene (dotted underlined), is indicated in upper case.