Table 1 Oligonucleotides used for the real-time PCR screening, DNA walking and PCR assays.
Assays | Methods | Oligonucleotide names | Oligonucleotide sequences | References |
|---|---|---|---|---|
Real-time PCR screening | p35S | p35S-F | AAAGCAAGTGGATTGATGTGATA | |
p35S-R | GGGTCTTGCGAAGGATAGTG | |||
Real-time PCR screening | pNOS | pNOS-F | CGTTTTACGTTTGGAACTGACA | |
pNOS-R | CTCATTAAACTCCAGAAACCCG | |||
Real-time PCR screening | tNOS | tNOS-F | GATTAGAGTCCCGCAATTATACATTTAA | |
tNOS-R | TTATCCTAGKTTGCGCGCTATATTT | |||
Real-time PCR screening | t35S pCAMBIA | t35S pCAMBIA-F | CGGGGGATCTGGATTTTAGTA | |
t35S pCAMBIA-R | AGGGTTCCTATAGGGTTTCGCTC | |||
Real-time PCR screening | Cry1Ab/Ac | Cry1Ab/Ac-F | ACCGGTTACACTCCCATCGA | |
Cry1Ab/Ac-R | CAGCACCTGGCACGAACTC | |||
Real-time PCR screening | t35S | t35S-F | In-house | Unpublished data |
t35S-R | In-house | Unpublished data | ||
Real-time PCR screening | CAP_CaMV | CAP-F | In-house | Unpublished data |
CAP-R | In-house | Unpublished data | ||
Real-time PCR screening | CRT2_CaMV | CRT2-F | CCAGAAGAACATTGGGTCAATGC | |
CRT2-R | CTATGTCTTTGCAGACTTTGCTGAT | |||
Real-time PCR screening | A1 | qPhA1_F4 | CGACTTCTGCCGTCGCG | |
qPhA1_R4 | GATGATGGTGACCAGGTCCAG | |||
DNA Walking | p35S-F | p35S-F a | GGGTCTTGCGAAGGATAGTG | |
p35S-F b | TGTGCGTCATCCCTTACGTCAGT | |||
p35S-F c | TATCACATCAATCCACTTGCTTT | |||
DNA Walking | p35S-R | p35S-R a | AAAGCAAGTGGATTGATGTGATA | |
p35S-R b | ACTGACGTAAGGGATGACGCACA | |||
p35S-R c | CACTATCCTTCGCAAGACCC | |||
PCR confirmation assay | GM petunia transgene flanking region | Junction-F | CATTTCGCCCTCATGAAAATGAT | This study |
Junction-R | GGTCGCCGCATACACTATTC | This study |
