Figure 5
From: GABAergic neurons in the olfactory cortex projecting to the lateral hypothalamus in mice
Mitral and tufted cells in the olfactory bulb send axonal projections to the VON. (a) Sagittal sections of the whole brain of mitral and tufted cells (M/TCs) in a tdTomato-labelled transgenic mouse after injection of retrograde adeno-associated virus (AAV) vector encoding EGFP into the lateral hypothalamus (LH). The inset of the left panel is magnified in the right panel. Note that vascular endothelial cells and a subset of neurons in the olfactory cortex were also labelled with tdTomato. Scale bars: 1 mm in left panel, 100 μm in right panel. (b) Sagittal sections of the ventral olfactory nucleus (VON) with immunostaining for DARPP-32 (blue). Dendrites of EGFP-labelled VON neurons (green) innervated layer Ia of the olfactory cortex which was innervated by axons of tdTomato-labelled M/TCs (red). Scale bar: 100 μm.
