Table 3 Gene-specific primers used for qPCR in adrenal and pituitary tissue.
Gene | Accession | Orientation | Primer Sequence (5′ - 3′) | Product Length (bp) |
|---|---|---|---|---|
LBR | NM_205342 | Forward | GGTGTGGGTTCCATTTGTCTACA | 80 |
Reverse | CTGCAACCGGCCAAGAAA | |||
MC2R | NM_001031515 | Forward | ATTGCCTCACTGCCAACCAG | 160 |
Reverse | AGCAGGCACAGTAAGGGTTG | |||
StAR | NM_204686.2 | Forward | AGTGATGGCCCTTATCTCGGT | 103 |
Reverse | GGTGGCTGCTACAAACACTGC | |||
HSD3B2 | NM_205118.1 | Forward | GCCAAAGAGGAGCAAACCAG | 203 |
Reverse | TTCACCTCAGTCTTGCCCTG | |||
POMC | NM_001031098 | Forward | ATTTTACGCTTCCATTTCGC | 141 |
Reverse | AATGGCTCATCACGTACTTGC | |||
CRHR1 | NM_204321 | Forward | CCCTGCCCCGAGTATTTCTA | 141 |
Reverse | CTTGCTCCTCTTCTCCTCACTG | |||
VT4R | NM_001110438 | Forward | GGTTGCAGTGTTTTCAGAGTCG | 137 |
Reverse | CAAGATCCGCACCGTCAAG |
