Figure 2 | Scientific Reports

Figure 2

From: Apoptotic endothelial cells release small extracellular vesicles loaded with immunostimulatory viral-like RNAs

Figure 2

ApoExos contain mainly non-ribosomal non-coding RNAs. (a) ApoExos are enriched in non-coding transcripts. RNA-Seq reads were quantified and pseudo-aligned on human Ensembl reference transcriptome using Kallisto. Transcripts were then classified according to their biotype. Pie charts represent the mean proportions of each transcript biotype (n = 2). rRNA: ribosomal RNA. (b,c) ApoExos are enriched in non-coding transcripts. Detailed distribution of long (b) and short (c) non-coding RNA transcripts subtypes (Two-tailed unpaired T test, *p ≤ 0.05, **p ≤ 0.01, n = 2). LincRNA: long intergenic non-coding RNA, miRNA: microRNA, mt-tRNA: mitochondrial transfer RNA, snoRNA: small nucleolar RNA, snRNA: small nuclear RNA, srpRNA: signal recognition particle RNA. (d) Top ten non-ribosomal non-coding RNAs in ApoExos. Relative abundance in ApoExos and apoptotic bodies expressed as log2 transcripts per million (TPM). (Two-tailed unpaired T test, *p ≤ 0.1, **p ≤ 0.05, n = 2).

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