Figure 5

LUV size is increased at a low Rh2/lipid ratio in the presence of eSM and the absence of Chol. LUVs composed of eSM:ePC (1:1, striped bars), ePC:Chol (1:1, grey bars) or eSM:ePC:Chol (1:1:1, black bars) were incubated for 5 min with 1% DMSO or increasing Rh2/lipid ratios at 25 °C and vesicle size was determined by dynamic light scattering. Data are means of triplicated assays. One-way ANOVA with Dunnett’s post tests to compare control versus Rh2-treated LUVs. **p < 0.01.