Figure 6
Membrane rigidity is increased at a low Rh2/lipid ratio in the presence of eSM and the absence of Chol. LUVs composed of eSM:ePC (1:1, striped bars), ePC:Chol (1:1, grey bars) or eSM:ePC:Chol (1:1:1, black bars) combined with Laurdan (0.5% mol, A) or DPH (0.5% mol, B) were incubated for 5 min with 1% DMSO or increasing Rh2/lipid ratios at 25 °C. Laurdan generalized polarization (GPex) and DPH fluorescence anisotropy (r) were measured following Rh2 treatment. The λex was respectively set at 340 nm (Laurdan) and 365 nm (DPH) and the λem at 440 and 490 nm (Laurdan) and 425 nm (DPH). Results are means of three independent experiments in triplicates. One-way ANOVA with Dunnett’s post tests to compare control versus Rh2-treated LUVs. *p < 0.05; **p < 0.01.
