Figure 9
Rh2 induces positive membrane curvature in the presence of eSM and small buds followed by intra-luminal vesicles in its absence. GUVs composed of eSM:ePC (A), ePC:Chol (B) and eSM:ePC:Chol (C) were labeled with Rhod-PE (red) and NBD-PE (green) to reveal the liquid-disordered (Ld) and liquid-ordered (Lo) phases, respectively. GUVs were incubated with 1% DMSO (control, 0 min) or Rh2/lipid ratio of 3 and visualized at the indicated times at room temperature with a Zeiss wide-field fluorescence microscope (Observer.Z1) using a plan-Apochromat 40x/1.4 oil Ph3 objective. White arrows point to solid-ordered phases (So), grey arrows to small buds, white arrowheads to inward membrane budding and grey arrowheads to intra-luminal vesicles. Scale bars, 20 µm. Images are representative of two independent experiments.
