Figure 4
From: A normothermic ex vivo organ perfusion delivery method for cardiac transplantation gene therapy
Luciferase protein expression in transplanted heart. (A) Lane1 Native LV, Lane 2 Native RV, Lane 3 Liver, Lane 4 native Septum, Lane 5 allograft LV, Lane 6 allograft RV, Lane 7 allograft septum, Lane 8. Naïve pig LV. (B) Luciferase activity from tissues corresponding to Fig. 4a lanes 1–8. (C–F) Immunostaining for Luciferase protein. (C) Native coronary, (D). Allograft coronary, (E) native LV, (F). Allograft LV. (Note: (A), the gel represents two different gels. For the image, these two gels were combined between lanes 4 and 5 and an additional ladder and a positive control lane with HeLa cells transfected with Ad-Luc lane removed for better display).
