Table 1 Composition of perfusate for OCS machine.

From: A normothermic ex vivo organ perfusion delivery method for cardiac transplantation gene therapy

Component

Concentration/ML of Total Circuit

Blood Collection and Autotransfusion −750 ML

Post autotransfusion yield

n/a

Blood Reconstitution 260 ML

Plasmalyte 200 ml

n/a

Albumin

7.7 mg/ml

Heparin

6.15 iu/ml

Circuit Prime 604 ML

Transmedics priming solution 500 ml

n/a

Albumin

7.7 mg/ml

Ciprofloxacin

0.06 mg/ml

Cefazolin

0.62 mg/ml

Adult Multi-V

1 unit

Solumedrol

0.15 mg/ml

Sodium Bicarbonate

0.012 mEq/ml

Corrective Medications 12 ML

Calcium Gluconate

0.37 mg/ml

Dextrose

0.615 mg/ml

Sodium Bicarbonate

0.003 mEq/ml

  1. Final volume of the circuit was 1626 ml.