Table 2 TSP3 active site mutant halo assay results.

From: Structure and tailspike glycosidase machinery of ORF212 from E. coli O157:H7 phage CBA120 (TSP3)

Wella

Mutation

Halo Formationb

1

Wild-type

++

2

E362Q

+

3

D383N

++

4

D426N

+

5

E362Q and D383N

+

6

E362Q and D426N

−

7

D383N and D426N

+

8

E362Q, D383N, and D426N

−

9

PBS Control

−

  1. aWell numbering corresponds to Fig. 6.
  2. bSymbols are as follows: −, No observable halo;+, 0–1.5 cm halo diameter; ++, >1.5 cm halo diameter.
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