Figure 6
From: Low-cost, sub-micron resolution, wide-field computational microscopy using opensource hardware
(a) Frequency space of a brightfield image obtained using an oblique illumination angle. Blue and green dots indicate initial and corrected LED positions respectively by using circle fitting. (b1) Aberrations recovered from each section are used as initial estimates for neighboring sections, starting from the center of the FOV towards the edges. (b2,b3) Examples of recovered aberrations throughout the full-FOV indicating spatially-varying aberrations. (c) Implementing LED calibration on each segment across the FOV enabled us to find the spatially varying distortion by measuring the global LED position shift.
