Table 3 Assessment summary of the different RNA preparation kits and conditions analyzed in this study.
Kit/Input | NR | MR | DR | GD | DEG | AC |
|---|---|---|---|---|---|---|
Illumina technology | ||||||
mRNATruseq_1 ug | ***** | ***** | ***** | ***** | ***** | Yes |
ssmRNATruseq_1 ug | ***** | ***** | ***** | ***** | ***** | Yes |
ssTotRNATruseq_1 ug | ***** | ***** | ***** | ***** | ***** | Yes |
ssmRNATruseq_100 ng | ***** | ***** | *** | **** | ***** | Yes |
ssTotRNATruseq_100 ng | ***** | ***** | ***** | ***** | ***** | Yes |
SMARTer technology | ||||||
RiboG_ssTotSmarter_100 ng | **** | * | *** | **** | *** | Yes |
RiboZ_ssTotSmarter_100 ng | ** | ** | ** | *** | ** | No |
RiboZ_ssTotSmarter_10 ng | * | ** | * | *** | * | No |
SMARTer Ultra-Low technology | ||||||
mRNASmarterUL_XT1 ng_750 pg | *** | **** | NA | *** | *** | Yes |
mRNASmarterUL_XT1 ng_130 pg | *** | **** | NA | *** | *** | Yes |
