Figure 5

mts knockdown causes reduction of Vtd level in S2 cells and wing imaginal disc. (a) Immunoblot using extracts from S2 cells treated with control dsRNA (pSK vector) or mts dsRNA. Mts level is significantly reduced from Day 4. Vtd level is also reduced from Day 4. Immunoblot with anti-Tubulin and anti-Histone H3 staining is used as controls to compare the amount of Mts or Vtd. (b–b”’,c–c”’) Wing imaginal discs were stained by DAPI (blue), anti-Vtd (red) antibodies. (b–b”’) GFP⁺ cells are mts knockdown clones induced by hs-FLP; Ay > mts RNAi. mts RNAi clones show reduction of Vtd. Scale bar, 20 µm. (c–c”’) tws⁶⁰ mutant clones marked by the lack of GFP expression show reduction of Vtd staining. Scale bar, 20 µm. (d) Immunoblot using extract from w¹¹¹⁸ wing imaginal disc (control) or tws⁶⁰ homozygous wing imaginal disc (left panel). Quantitative analysis (right panel). Vtd levels are normalized to the amount of Histone H3. Error bars are s.d. n = 3. **P < 0.01 (t-test). (a,d) Full-length blots are presented in Supplementary Fig. 6.