Figure 3
From: Proteomic insight into the pathogenesis of CAPN5-vitreoretinopathy
Gene ontology and pathway analysis reveals differences among early and late CAPN5-NIV: (A) Differentially-expressed proteins from early and late CAPN5-NIV compared to controls. Gene ontology analysis categorized each protein group by biological process, molecular function, and cellular compartment. (B,C) Differentially-expressed proteins from early CAPN5-NIV compared to controls. Results are represented as a volcano plot. The horizontal axis (x-axis) displays the log2 fold-change value (early CAPN5-NIV vs. controls) and the vertical axis (y-axis) displays the noise-adjusted signal as the -log10 (p-value) from the 1-way ANOVA analysis. Proteins with log2 fold-changes greater than 15 (upregulated) are colored cyan (early) and orange (late) while proteins with log2 fold-changes lower than −15 are represented in green (downregulated). (D) Comparative analysis of upregulated proteins (compared to controls) using Venn diagrams. A total of 74 upregulated proteins are shared among the two stages compared to controls (p < 0.05). (E) Comparative analysis of downregulated proteins using Venn diagrams. A total of 49 downregulated proteins are shared among the two stages compared to controls (p < 0.05). (F) Top ten pathways represented in CAPN5-NIV. Pathways are ranked by their −log (p-value) obtained from the right-tailed Fisher’s Exact Test.
