Figure 3
From: Rapamycin modulates tissue aging and lifespan independently of the gut microbiota in Drosophila
Rapamycin treatment maintains proteostasis during aging in an autophagy-dependent manner. (A,B) Immunostaining of indirect flight muscles from 10 and 42 day old daGS > UAS-Atg1-RNAi female flies with or without both rapamycin and RU486 treatment from day 4 onwards (post-eclosion), showing polyubiquitinated aggregates (green channel, anti-FK2) and muscles (red channel stained with phalloidin/F-Actin). Scale bar is 10 µm. (C,D) Quantifications of polyubiquitin aggregates in muscle as shown in (A,B) respectively. **p < 0.01, one-way ANOVA w/ Newman-Keuls post-test; n > 12, one fly per replicate. Rapamycin was provided in the media at a concentration of 10 μg/ml and RU486 at a concentration of 25 μg/ml for (A–D). All error bars represent SEM.
