Figure 3
From: BRAF inhibition sensitizes melanoma cells to α-amanitin via decreased RNA polymerase II assembly
α-amanitin induces massive cell death in PLX4720-treated melanoma cell lines. (A–C) Representative immunoblots for POLR2A and apoptosis markers in indicated melanoma cell lines treated with α-amanitin (1 μg/ml in A375-P and 0.25 μg/ml in UACC-62 and WM-266-4) alone or in combination with 1 μM PLX4720 (n = 3). P-MEK, total MEK and α-Tubulin served as a treatment and loading control. (D–F) Apoptosis measurements of indicated melanoma cell lines by Annexin V-FITC and propidium iodide double staining after treatment with α-amanitin alone or in combination with PLX4720. Percentage of Annexin V positive cells for each condition is displayed. Data are mean ± SD (n = 3 biological replicates).
