Figure 4

Changes in the expression of intercellular junctions during the senescence of PMCs in vitro. The results of the quantitative examination of SA-β-Gal, connexin 43, E-cadherin, occludin, and desmoglein in young (Young) and senescent (Sen) PMCs. (a) Representative pictures showing the decreased expression of junctional proteins in the senescent PMCs. (b) Experiments were performed with 8 cultures of PMCs obtained from different donors. The results are expressed as the mean ± SEM. *P < 0.05; **P < 0.01; ***P < 0.001 vs. Young. RFU – Relative Fluorescence Units (magnification ×200).