Figure 4

P. chabaudi-induced liver inflammation is reduced in the absence of IL-1α. C57BL/6 and Il1a^−/− mice were analyzed at day 7 p.i. with 1 × 10⁶P. chabaudi-iRBCs. Non-infected mice (day 0) were used as controls. (A) The number of total leukocytes and CD4⁺, CD8⁺, CD4^-CD8^-NK1.1⁺, CD11c⁺, CD11b⁺ Ly6C^high and CD11b⁺Ly6G⁺ cells in the liver. The data are expressed as the means ± SD (n = 3–5) of one representative experiment out of three. (B) Contour plots of IFN-γ by CD4⁺ cells and TNF-α production by myeloid cells (CD11b⁺Ly6G⁺ and CD11b⁺Ly6C^high). (C) Numbers of IFN-γ⁺CD4⁺, TNF-α⁺CD11b⁺Ly6C^high and TNF-α⁺CD11b⁺Ly6G⁺ cells per liver. (D) The relative Tnfa, Il6 and Ifng mRNA expression in the liver tissue. The data were pooled from two independent experiments (n = 3–8). Significant differences were observed between C57BL/6 and Il1a^−/− mouse groups with *p < 0.05, **p < 0.01 and ***p < 0.001, using the Mann-Whitney test.