Figure 5

Inflammatory cells are a major source of IL-1α in the liver during P. chabaudi malaria. C57BL/6 mice were infected with 1 × 10⁶ P. chabaudi-iRBCs. Non-infected mice (day 0) were used as controls. (A) Immunofluorescence staining for IL-1α (green), F-actin (red) and nucleus (DAPI, blue) in the liver sections (40x magnification, scale bars correspond to 20 μm). (B) Quantification of the number of IL-1α⁺ cells per field in the liver sections. The data are expressed as the means ± SD (n = 3–5) of one representative experiment out of two. (C) Contour plots of IL-1α production by CD45⁻ and CD45⁺ cells (gated on live cells). (D) The number of IL-1α⁺CD45⁺ cells distributed in CD11b⁻ and CD11b⁺ cell populations. The data are expressed as the means ± SD (n = 3–5) of one representative experiment out of two. Significant differences were observed between indicated groups with *p < 0.05 and ***p < 0.001, using the Mann-Whitney test.