Figure 6

Neutrophils are a major source of IL-1α in the liver during P. chabaudi malaria. C57BL/6 mice were analyzed at day 7 p.i. with 1 × 10⁶P. chabaudi-iRBCs. Non-infected mice (day 0) were used as controls. (A) Contour plots showing F4/80⁺ (light blue) and CD11b⁺ (grey) cells, The later population was subdivided in monocytes (Ly6C^highLy6G⁻ - dark blue), neutrophils (Ly6C^intLy6G⁺ - green) and other CD11b⁺ cells (Ly6C⁻Ly6G⁻ - red), IL-1α production was evaluated in these populations. The numbers of Ly6C^highLy6G⁻, Ly6C^intLy6G⁺ and Ly6C⁻Ly6G⁻ cells per liver, and the numbers of IL-1α⁺ cells inside each population are shown. (B) Immunofluorescence staining for IL-1α (green), Ly6G (red) and CD45 (gray) or IL-1α (green) and F4/80 (red) (40x magnification, scale bar corresponds to 16 μm).