Figure 1
Longitudinal imaging using hrGRIN lens system. Chronically implanted hrGRIN lens assemblies can be used to image the same cellular features over the course of several weeks using multiphoton microscopy. (a) Images were acquired at 3 weeks post-implantation and then subsequently at 4 weeks, 8 weeks, and 12 weeks. Common features are identified across imaging sessions, such as two axons running parallel to one another (solid arrow) and a bundle of axons resembling crow’s feet (dashed arrow). (b) Texas Red dye conjugated to dextran was injected via the tail vein to demonstrate the ability to use injectable dyes as a fiduciary marker (red). Each image is a z-projection of five image planes, separated by 2 µm. Scale bar denotes 10 µm for all images.
