Figure 5
Progression of secondary damage. (a) Over the course of 30 days, mFPI mice had a significantly higher percentage of axons with varicosities (p < 0.0001) and axons developing terminal bulbs (p = 0.0017). (b,c). The percentage of newly damaged axons was compared, including new axons with varicosities, undulated axons progressing to axons with varicosities, axons with varicosities progressing to terminal bulbs, and terminal bulbs resulting in axon loss, that contributed to the total percentage of damaged axons at each time-point. As expected, the mFPI mice had a significantly higher percentage of axons in the field of view that were newly damaged (p < 0.0001 and p = 0.004, respectively) at the 1-hr and 3-days post-injury time points compared to sham-injured mice. Isolated swellings occasionally and transiently appear in the adult rodent brain, which likely explains their appearance in sham animals23. Two-way ANOVA with Sidak correction. ****p < 0.0001, **p < 0.01 vs. sham-injured.
