Figure 4 | Scientific Reports

Figure 4

From: Sildenafil Reduces Neointimal Hyperplasia after Angioplasty and Inhibits Platelet Aggregation via Activation of cGMP-dependent Protein Kinase

Figure 4

Effects of sildenafil on cGK activity. (a) Enzyme immunoassay for cGMP shows that the concentration of cGMP increased in VSMCs with sildenafil treatment. P values is 0.05 between two groups (n = 3). (b) RT-PCR shows that sildenafil treatment did not change the expression cGK Ια and cGK Ιβ. cGK inh. = cGK inhibitor (n = 3). (c) Western blot analysis indicates that sildenafil treatment increased VASP phosphorylation (a substrate of cGK), but did not change the level of cGK itself. The phosphorylation of VASP by sildenafil was reversed by cGK inhibitor. pVASP Ser 239 = phospho-VASP at serine239 (n = 3). (d) Immunofluorescence staining for pVASP shows the similar effect with the previous western blot. pVASP239 = phospho-VASP at serine239 (n = 3). Scale bar = 50 µm.

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