Figure 7 | Scientific Reports

Figure 7

From: Ras suppressor-1 (RSU-1) promotes cell invasion in aggressive glioma cells and inhibits it in non-aggressive cells through STAT6 phospho-regulation

Figure 7

RSU-1 silencing enhanced STAT6 phosphorylation in non-aggressive glioma cells while diminished STAT6 phosphorylation in aggressive glioma cells. (A) The heatmap depicts mean fold change results from the phosphoproteomic analysis performed for 21 phospho-proteins in two independent experiments between the treated (RSU-1 siRNA) and control cells (NSC siRNA) for both H4 and A172 cell lines. Red arrow indicates the most significant change in phosphorylation status upon RSU-1 knockdown. (B) Quantification of the phosphoprotein analysis data for P-STAT6 following RSU-1 silencing using NSC as the control sample. (C) Representative immunoblot validating the phosphorylation status of STAT6 for the same protein samples as in (A). Cropped blots are from samples run on the same gel and original pictures of the western blots are displayed in Supplementary Fig. 8. Asterisks denote a statistically significant difference (p < 0.05) compared to NSC.

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