Figure 8 | Scientific Reports

Figure 8

From: Ras suppressor-1 (RSU-1) promotes cell invasion in aggressive glioma cells and inhibits it in non-aggressive cells through STAT6 phospho-regulation

Figure 8

In vitro effects of the phospho-STAT6 inhibitor (AS1517499), in glioma cell (H4 and A172) invasion. (A) Representative images of transwell invasion assay performed following treatment with phospho-STAT6 inhibitor, AS1517499 (at 100, 200 or 300 nM) or DMSO for 24 h. Cells were left to invade for an additional 24 h time period with inhibitor. The invading cells were counted in nine (9) randomly chosen microscopic fields per transwell. Scale bar: 100 μm. (B) Total number of invaded cells compared to DMSO for each cell line per transwell. Two transwells were included per sample and at least two (2) independent experiments were performed. (C) Representative images of Western blot results of STAT6 phosphorylation in glioma cell lines (H4 and A172) following treatment with DMSO or 300 nM of AS1517499. Original pictures of the western blots are displayed in Supplementary Fig. 10. Asterisks denote a statistically significant difference (p < 0.05) compared to DMSO. (D) Schematic diagram illustrating the significant findings of this work.

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