Figure 4

Mutant Phl p 7 displays a reduced negative surface charge. Electrostatic surface potential (A) and ribbon diagram (B) of Phl p 7 wildtype with calcium (PDB code: 2lvk), without calcium (PDB code: 2lvi) and mutant Phl p 7 (homology model based on 2lvi). Calcium-binding loops and modified amino acids are marked (Asp 12, Asn 14, Asp 47 and Asp 49). (A) The color scheme of the electrostatic surface potential ranges from red via white to blue for −10 to +10 kT/e. (B) Ribbon diagrams show helices (red), loop regions (green), calcium-interacting residues (yellow), and bound calcium (grey). All structures are aligned and in the same orientation.