Figure 1 | Scientific Reports

Figure 1

From: Latent Infection of Valsa mali in the Seeds, Seedlings and Twigs of Crabapple and Apple Trees is a Potential Inoculum Source of Valsa Canker

Figure 1

The amplification curve (A) and standard curve of qPCR for gradient dilution of V. mali. genomic DNA. (A) The amplification curve represents the DNA with the concentrations ranging from 264 ng µL−1 to 0.264 fg µL−1. (B) The log amount of the sample copy number calculated by the DNA concentration was plotted versus the Ct value, and the equation of the regression line and the correlation coefficient (R2) are displayed in the graph. The standard curve was generated using gradient dilution genomic DNA corresponding to 1.95 to 1.95×107 V. mali strain sample copies per microlitre. Each concentration of DNA was repeated three times. Error bars represent standard deviation from three replicate reactions.

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