Figure 3 | Scientific Reports

Figure 3

From: Latent Infection of Valsa mali in the Seeds, Seedlings and Twigs of Crabapple and Apple Trees is a Potential Inoculum Source of Valsa Canker

Figure 3

Multiple sequence alignment of the 142 bp partial eukaryotic elongation factor 1 alpha (EF-1ɑ) gene sequences. Polymorphic sites were used to design specific primers VF1F/VE1R for V. mali. The accession numbers JQ900318.1, JQ900322.1 and JQ900317.1 are the partial EF-1ɑ genes of Valsa mali var. mali; JQ900326.1 and JQ900332.1 are the partial EF-1ɑ genes of Valsa mali var. pyri; JQ900337.1 and JQ900336.1 are the partial EF-1ɑ genes of Valsa malicola; JQ900338.1 is the partial EF-1ɑ gene of Cytospora chrysosperma; and JQ900340.1 is the partial EF-1ɑ gene of Leucostoma persoonii. The primer sequences are indicated (VE1F as a forward complement and VE1R as a reverse complement). Conserved sequences between the Valsa mali var. mali strains are shaded in grey, and mismatches with other species or genus strain sequences are indicated with dark shading.

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