Table 1 Strains and plasmids used.
Name | Characteristics | Reference |
|---|---|---|
Bacterial strains | ||
MG1655 | F-lambda-rph-1 | Lab stock |
BW27783 | Δ(araFGH) ϕ(ΔaraEpPCP8-araE) | 17 |
MEK65 | MG1655 recB::halotag | This work |
MEK705 | MG1655 recB::sfGFP | This work |
MEK573 | MG1655 recC::halotag | This work |
MEK711 | MG1655 recC::sfGFP | This work |
MEK556 | MG1655 recB::halo::cohalo | This work |
Plasmids | ||
pSF1 | Expression of HaloTag under the control of the pBAD promoter | This work |
pBH35 | Template for plasmid mediated recombination to construct recB::halotag fusion | This work |
pBH29 | Template for plasmid mediated recombination to construct recB::sfGFP fusion | This work |
pHT8 | Template for plasmid mediated recombination to construct recB::halo::cohalo fusion. | This work |
